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Team:Imperial College London/M1/Genetic
From 2009.igem.org
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LacI is produced constitutively by the E. coli bacterium, and it represses the pLambda promoter. Thus, our proteins of interest, PAH or cellulase will not be produced. | LacI is produced constitutively by the E. coli bacterium, and it represses the pLambda promoter. Thus, our proteins of interest, PAH or cellulase will not be produced. | ||
| - | To start the drug manufacturing process, IPTG is pipetted into the system. IPTG will repress LacI. As a result, protein production (Module 1) is de-repressed. PAH or cellulase will be produced by the <i>E.ncapsulator</i>. | + | To start the drug manufacturing process, sufficient IPTG is pipetted into the system. IPTG will repress LacI. As a result, protein production (Module 1) is de-repressed. PAH or cellulase will be produced by the <i>E.ncapsulator</i>. |
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Revision as of 14:46, 10 September 2009
M1 Genetic Circuit
This is the genetic circuit that constitutes protein production (Module 1).
LacI is produced constitutively by the E. coli bacterium, and it represses the pLambda promoter. Thus, our proteins of interest, PAH or cellulase will not be produced.
To start the drug manufacturing process, sufficient IPTG is pipetted into the system. IPTG will repress LacI. As a result, protein production (Module 1) is de-repressed. PAH or cellulase will be produced by the E.ncapsulator.
