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Team:Imperial College London/M1/Genetic
From 2009.igem.org
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| - | This is the genetic circuit | + | This is the genetic circuit responsible protein drug fabrication (Module 1). |
| - | LacI is produced constitutively by the E. coli bacterium, and it represses the pLambda promoter. | + | LacI is produced constitutively by the E. coli bacterium, and it represses the pLambda promoter. This inhibits production of the proteins of interest (PAH and cellulase). |
| - | To start the drug manufacturing process, | + | To start the drug manufacturing process, IPTG is pipetted into the system. IPTG will repress LacI. As a result, protein production is de-repressed. Then, the enzymes PAH or cellulase will be produced by the <i>E.ncapsulator</i>. |
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Revision as of 13:34, 11 September 2009
M1 Genetic Circuit
This is the genetic circuit responsible protein drug fabrication (Module 1).
LacI is produced constitutively by the E. coli bacterium, and it represses the pLambda promoter. This inhibits production of the proteins of interest (PAH and cellulase).
To start the drug manufacturing process, IPTG is pipetted into the system. IPTG will repress LacI. As a result, protein production is de-repressed. Then, the enzymes PAH or cellulase will be produced by the E.ncapsulator.
