Template:Team:KULeuven/10 September 2009/BlueLightReceptor

(Difference between revisions)

Revision as of 16:47, 10 September 2009

since the results from ourt light experiments keep failing, some new ideas were computed. pSB1A2 is the backbone for our construct but since this is a high copy number plasmid perhaps the ratio of promotor to repressor is unequal (not enough repressor). A new enting in LC was done with ligX. these were grown for a few hour at 37°C and then put in 16°C room overnight so that more repressor can be made (its promotor is temperature-sensitive).
ligation from 9/08 was electroporated in competent cells.
miniprep of LigX, restriction digest and gel electrophoresis. the signals were good so a motherplate for ligX was made. also, the vector pSB1A2 was extracted from the gel (39,1 ng/µl).

nanodrop results:

@@ Line 1: / Line 1: @@
-# miniprep of LigX
+# since the results from ourt light experiments keep failing, some new ideas were computed. pSB1A2 is the backbone for our construct but since this is a high copy number plasmid perhaps the ratio of promotor to repressor is unequal (not enough repressor). A new enting in LC was done with ligX. these were grown for a few hour at 37°C and then put in 16°C room overnight so that more repressor can be made (its promotor is temperature-sensitive).
+# ligation from 9/08 was electroporated in competent cells.
+# miniprep of LigX, restriction digest and gel electrophoresis. the signals were good so a motherplate for ligX was made. also, the vector pSB1A2 was extracted from the gel (39,1 ng/µl).
 nanodrop results:
 {| border ="1" align="center"