Template:Team:KULeuven/6 September 2009/VanillinProduction
From 2009.igem.org
(Difference between revisions)
Line 1: | Line 1: | ||
- | * Restriction digest overnight of | + | * Restriction digest overnight of ''sam5'' (1,2) and ''sam8'' (1,2) |
* Growth of EF Ligation (woohoo!) | * Growth of EF Ligation (woohoo!) | ||
- | ** | + | ** Transferred 4 colonies from EF ligation to a new plate |
- | ** Put the 4 | + | ** Put the 4 transferred colonies on liquid medium |
- | + | A miniprep and restriction digest (ON) of ''sam5, sam8, ech'' and ''fcs'' was performed on Saturday. On Sunday, a gel was run to check the inserts. These were good so a motherplate for all the genes could be made. |
Latest revision as of 12:04, 14 September 2009
- Restriction digest overnight of sam5 (1,2) and sam8 (1,2)
- Growth of EF Ligation (woohoo!)
- Transferred 4 colonies from EF ligation to a new plate
- Put the 4 transferred colonies on liquid medium
A miniprep and restriction digest (ON) of sam5, sam8, ech and fcs was performed on Saturday. On Sunday, a gel was run to check the inserts. These were good so a motherplate for all the genes could be made.