Template:Team:KULeuven/11 September 2009/BlueLightReceptor

(Difference between revisions)

Latest revision as of 08:20, 14 September 2009

The electroporated ligY did not grow. A new restriction digest on pcr product was performed with EcoRI and PstI.This was put on gel. There was a good signal so it was used to perform a ligation with vector pSB1A2.
A liquid culture that was made from pSB3K3 a few days ago showed growth after all. This was miniprepped and restriction digested to check whether the vector is actually present.
The liquid cultures with our blue light construct (lig) that stood overnight at 16°C was FACS-ed. Half of the cultures were exposed to blue light during the day and put under the FACS in the evening.

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-# the electroporated ligY did not grow. A new restrictie digest on pcr product {{kulpart|BBa_K238013}} was performed with EcoRI and PstI.This was put on gel. there was a good signal so it was used to perform a ligation with vector pSB1A2.
+# The electroporated ligY did not grow. A new restriction digest on pcr product {{kulpart|BBa_K238013}} was performed with EcoRI and PstI.This was put on gel. There was a good signal so it was used to perform a ligation with vector pSB1A2.
-# a liquid culture that was made from pSB3K3 a few days ago showed growth after all. this was miniprepped and restrictie digested to check wether the vector is actually present.
+# A liquid culture that was made from pSB3K3 a few days ago showed growth after all. This was miniprepped and restriction digested to check whether the vector is actually present.
-# the liquid cultures with our blue light construct (lig) that stood overnight at 16°C was FACS-ed. half of the cultures were exposed to blue light during the day and put under the FACS in the evening.
+# The liquid cultures with our blue light construct (lig) that stood overnight at 16°C was FACS-ed. Half of the cultures were exposed to blue light during the day and put under the FACS in the evening.