Team:Calgary/24 June 2009
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Jamie.feng (Talk | contribs) |
Jamie.feng (Talk | contribs) |
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*From this gel, it looks like our gene if interest, LuxOD47E is there as we see bads around 1.4 KB, as expected (gene is 1362 base pairs). We will proceed by isoltaing plasmid from the PCR product and performing a NotI Verification digest. | *From this gel, it looks like our gene if interest, LuxOD47E is there as we see bads around 1.4 KB, as expected (gene is 1362 base pairs). We will proceed by isoltaing plasmid from the PCR product and performing a NotI Verification digest. | ||
*So I pooled the DNA from the BBK amplification PCR. Lanes: 2,3,9,11 and 12 went into tube 1 and lanes 4,5,6,7 and 8 went into tube 2. I did a PCR product isolation (Sigma) according to the manufacturer's instructions and took the concentration using the Nanodrop Spectrophotometer. | *So I pooled the DNA from the BBK amplification PCR. Lanes: 2,3,9,11 and 12 went into tube 1 and lanes 4,5,6,7 and 8 went into tube 2. I did a PCR product isolation (Sigma) according to the manufacturer's instructions and took the concentration using the Nanodrop Spectrophotometer. | ||
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Revision as of 05:34, 18 September 2009
UNIVERSITY OF CALGARY