Team:Imperial College London/Drylab/Protein production/Analysis

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*In this model we have neglected the degradation rate of IPTG and also of the [IPTG-LacI] complex.
*In this model we have neglected the degradation rate of IPTG and also of the [IPTG-LacI] complex.
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==The model==
 
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We can describe the behavior of the system with 4 ODEs. In this system, the first two equations describe the expression of LacI protein, and these will be linked to how it represses production of the protein of interest.
 
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*In the absence of IPTG the system becomes:
 
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[Introduce system in the absence of IPTG here]
 
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Click here for a detailed explanation
 
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[https://2009.igem.org/Team:Imperial_College_London/Drylab/M1/Protein_production/Analysis/Detailed here]
 
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*When IPTG is introduced the system becomes:
 
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[Introduce full system with IPTG included] and expansion to the model.
 
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[link]
 
==Model Predictions==
==Model Predictions==
{{Imperial/09/TemplateBottom}}
{{Imperial/09/TemplateBottom}}

Revision as of 14:26, 18 September 2009



Model Assumptions

  • LacI assumptions:
    • The lac operon represses by constitutively synthesizing the LacI gene product [ref1].
    • Here we have assumed that all the other complicated dynamical pathways are at dynamic equilibrium [ref2], and we are only considering the LacI gene.
      • If these effects were included, the dynamics of the entire system will be more complicated, and the outcome of the model will be different.
    • In a culture grown overnight, levels of LacI expression will have reached steady state. We can assume that this is the case before we add in IPTG.


  • IPTG assumptions:
  • LacI and IPTG undergo a secondary set of reactions when IPTG is added in. We assume that 1 molecule of IPTG binds to 1 molecule of LacI, creating an intermediate reaction complex:
II09 M1 reaction.jpg
  • We also assume that the concentration of IPTG we put in is below the threshold of toxicity that could significantly start killing our cells.
    • [http://openwetware.org/wiki/IGEM:IMPERIAL/2009/M1/Modelling/Analysis/literatureIPTG Literature review on IPTG]
  • In this model we have neglected the degradation rate of IPTG and also of the [IPTG-LacI] complex.


Model Predictions

Mr. Gene   Geneart   Clontech   Giant Microbes

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