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Team:Imperial College London/Drylab/Protein production/Analysis
From 2009.igem.org
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*LacI and IPTG undergo a secondary set of reactions when IPTG is added in. We assume that 1 molecule of IPTG binds to 1 molecule of LacI, creating an intermediate reaction complex: | *LacI and IPTG undergo a secondary set of reactions when IPTG is added in. We assume that 1 molecule of IPTG binds to 1 molecule of LacI, creating an intermediate reaction complex: | ||
[[Image:II09_M1_reaction.jpg|centre]] | [[Image:II09_M1_reaction.jpg|centre]] | ||
| - | *We also assume that the concentration of IPTG we put in is below the threshold of toxicity that could significantly start killing our cells. | + | *We also assume that the concentration of IPTG we put in is below the threshold of toxicity that could significantly start killing our cells. ([http://openwetware.org/wiki/IGEM:IMPERIAL/2009/M1/Modelling/Analysis/literatureIPTG Literature review on IPTG]) |
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*In this model we have neglected the degradation rate of IPTG and also of the [IPTG-LacI] complex. | *In this model we have neglected the degradation rate of IPTG and also of the [IPTG-LacI] complex. | ||
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==Model Predictions== | ==Model Predictions== | ||
{{Imperial/09/TemplateBottom}} | {{Imperial/09/TemplateBottom}} | ||
Revision as of 14:41, 18 September 2009
- Overview
- The model
- Simulations
Model Assumptions
- LacI assumptions:
- The lac operon represses by constitutively synthesizing the LacI gene product [ref1].
- Here we have assumed that all the other complicated dynamical pathways are at dynamic equilibrium [ref2], and we are only considering the LacI gene.
- If these effects were included, the dynamics of the entire system will be more complicated, and the outcome of the model will be different.
- In a culture grown overnight, levels of LacI expression will have reached steady state. We can assume that this is the case before we add in IPTG.
- IPTG assumptions:
- LacI and IPTG undergo a secondary set of reactions when IPTG is added in. We assume that 1 molecule of IPTG binds to 1 molecule of LacI, creating an intermediate reaction complex:
- We also assume that the concentration of IPTG we put in is below the threshold of toxicity that could significantly start killing our cells. ([http://openwetware.org/wiki/IGEM:IMPERIAL/2009/M1/Modelling/Analysis/literatureIPTG Literature review on IPTG])
- In this model we have neglected the degradation rate of IPTG and also of the [IPTG-LacI] complex.
Model Predictions
