"
Team:Imperial College London/Wetlab/Protocols/Autoinduction
From 2009.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
{{Imperial/09/Tabs/Main/Wetlab/Protocols}} | {{Imperial/09/Tabs/Main/Wetlab/Protocols}} | ||
| + | {| style="color:#CCC; background-color:#325d97;" cellpadding="6" cellspacing="0" border="3" | ||
| <b>[[Team:Imperial_College_London/Wetlab/Protocols/SecondaryCarbon|Secondary Carbon Source Diauxie Growth]]</b> | | <b>[[Team:Imperial_College_London/Wetlab/Protocols/SecondaryCarbon|Secondary Carbon Source Diauxie Growth]]</b> | ||
| | | | ||
| Line 7: | Line 8: | ||
* To determine the best secondary carbon source for optimal growth | * To determine the best secondary carbon source for optimal growth | ||
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
| - | + | |} | |
Revision as of 14:46, 23 September 2009
| Secondary Carbon Source Diauxie Growth |
|
Glucose time delay
Aims
- Characterise the tunable time duration it takes before GFP expression (M2 activation)
Assay
- The cells will be grown until OD= 0.7.
- The RFP value will be monitered, although it is the GFP values that are more critical in this assay.
- OD and fluorescence data for GFP which can be converted in [http://partsregistry.org/cgi/measurement/new_batch.cgi Specific Promoter Units (SPUs)].
- The secondary carbon source will be taken from the previous experiment (see Secondary carbon source selection for CRP promoter)
- The IPTG concentrations will be taken from the previous experiment (see Determining concentration of IPTG)
See Glucose time delay for details
