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Team:Imperial College London/Wetlab/Protocols/Autoinduction
From 2009.igem.org
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! Overview and Aims | ! Overview and Aims | ||
| + | | <b>Promoter Characterisation</b> | ||
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| [[Team:Imperial_College_London/Wetlab/Protocols/SecondaryCarbon|Secondary Carbon Source Diauxie Growth]] | | [[Team:Imperial_College_London/Wetlab/Protocols/SecondaryCarbon|Secondary Carbon Source Diauxie Growth]] | ||
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* To determine the best secondary carbon source for optimal growth | * To determine the best secondary carbon source for optimal growth | ||
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| + | |||
| + | | <b>Auto-Induction</b> | ||
| + | | [[Team:Imperial_College_London/Wetlab/Protocols/Glucose delay|Glucose Time Delay]] | ||
| + | | * Characterise the tunable time duration it takes before GFP expression (M2 activation) | ||
| + | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
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Revision as of 14:56, 23 September 2009
| Section | Assay | Overview and Aims | Promoter Characterisation |
|---|---|---|---|
| Secondary Carbon Source Diauxie Growth |
| ||
| Auto-Induction | Glucose Time Delay | * Characterise the tunable time duration it takes before GFP expression (M2 activation) |
Glucose time delay
Aims
- Characterise the tunable time duration it takes before GFP expression (M2 activation)
Assay
- The cells will be grown until OD= 0.7.
- The RFP value will be monitered, although it is the GFP values that are more critical in this assay.
- OD and fluorescence data for GFP which can be converted in [http://partsregistry.org/cgi/measurement/new_batch.cgi Specific Promoter Units (SPUs)].
- The secondary carbon source will be taken from the previous experiment (see Secondary carbon source selection for CRP promoter)
- The IPTG concentrations will be taken from the previous experiment (see Determining concentration of IPTG)
See Glucose time delay for details
