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Team:Imperial College London/Wetlab/Protocols/Autoinduction
From 2009.igem.org
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! Overview and Aims | ! Overview and Aims | ||
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| <b>Promoter Characterisation</b> | | <b>Promoter Characterisation</b> | ||
| [[Team:Imperial_College_London/Wetlab/Protocols/SecondaryCarbon|Secondary Carbon Source Diauxie Growth]] | | [[Team:Imperial_College_London/Wetlab/Protocols/SecondaryCarbon|Secondary Carbon Source Diauxie Growth]] | ||
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* To generate the diauxie growth curves, as well as normal growth curves for Top-10 for modelling | * To generate the diauxie growth curves, as well as normal growth curves for Top-10 for modelling | ||
* To determine the best secondary carbon source for optimal growth | * To determine the best secondary carbon source for optimal growth | ||
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| <b>Auto-Induction</b> | | <b>Auto-Induction</b> | ||
| [[Team:Imperial_College_London/Wetlab/Protocols/Glucose delay|Glucose Time Delay]] | | [[Team:Imperial_College_London/Wetlab/Protocols/Glucose delay|Glucose Time Delay]] | ||
| * Characterise the tunable time duration it takes before GFP expression (M2 activation) | | * Characterise the tunable time duration it takes before GFP expression (M2 activation) | ||
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Revision as of 14:59, 23 September 2009
| Section | Assay | Overview and Aims |
|---|---|---|
| Promoter Characterisation | Secondary Carbon Source Diauxie Growth |
|
| Auto-Induction | Glucose Time Delay | * Characterise the tunable time duration it takes before GFP expression (M2 activation) |
Glucose time delay
Aims
- Characterise the tunable time duration it takes before GFP expression (M2 activation)
Assay
- The cells will be grown until OD= 0.7.
- The RFP value will be monitered, although it is the GFP values that are more critical in this assay.
- OD and fluorescence data for GFP which can be converted in [http://partsregistry.org/cgi/measurement/new_batch.cgi Specific Promoter Units (SPUs)].
- The secondary carbon source will be taken from the previous experiment (see Secondary carbon source selection for CRP promoter)
- The IPTG concentrations will be taken from the previous experiment (see Determining concentration of IPTG)
See Glucose time delay for details
