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Team:Imperial College London/Stomach
From 2009.igem.org
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possible cleavage sites mapped on it and /or a table of cleavage site positions. | possible cleavage sites mapped on it and /or a table of cleavage site positions. | ||
| - | + | <form method=POST action="http://www.expasy.ch/cgi-bin/peptidecutter/peptidecutter.pl" href="_Blank"> | |
| - | <form method=POST action="http://www.expasy.ch/cgi-bin/peptidecutter/peptidecutter.pl"> | + | |
Enter a UniProtKB (Swiss-Prot or TrEMBL) protein | Enter a UniProtKB (Swiss-Prot or TrEMBL) protein | ||
identifier, ID (e.g. ALBU_HUMAN), or accession number, AC (e.g. P04406), | identifier, ID (e.g. ALBU_HUMAN), or accession number, AC (e.g. P04406), | ||
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<input type="submit" value="Perform"> the cleavage of the protein. | <input type="submit" value="Perform"> the cleavage of the protein. | ||
<input type="reset" value="Reset"> the fields.<P> | <input type="reset" value="Reset"> the fields.<P> | ||
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<input type=hidden name=enzyme_number value= less_enzymes checked> | <input type=hidden name=enzyme_number value= less_enzymes checked> | ||
<input type=hidden name=enzyme value=Pn1.3 checked> | <input type=hidden name=enzyme value=Pn1.3 checked> | ||
<input type=hidden checked name=cleavage_map value=cleavage_map> | <input type=hidden checked name=cleavage_map value=cleavage_map> | ||
| + | |||
| + | <!-- | ||
| + | Map of cleavage sites. Please select the number of amino acid within one block: | ||
| + | <select name="block_size" size=1> | ||
| + | <option selected value="60">60 | ||
| + | </select><br> | ||
| + | |||
| + | --> | ||
<input type=hidden name=alphtable checked value=alphtable> | <input type=hidden name=alphtable checked value=alphtable> | ||
<input type=hidden name=cleave_number checked value=all> | <input type=hidden name=cleave_number checked value=all> | ||
</form> | </form> | ||
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Revision as of 10:52, 2 October 2009
Contents |
Human Digestive Proteases:
The stomach serves to break large proteins into peptides. These peptides are then broken down into amino acids once they enter the duodenum.
- Pepsin = stomach
- Trypsin = duodenum
- Chymotrypsin
- Carboxypeptidase
Stomach
The stomach is the first point at which polypeptides are broken down. The low pH of the stomach causes enzymes to denature, opening them up to attack from proteases such as pepsin.
Pepsin
- Released by chief cells in the stomach.
- Expressed as a zymogen pepsinogen.
- Pepsinogen is converted to pepsin by HCl which is released by parietal cells of the stomach.
- Cleaves at the N-terminus after aromatic amino acids such as phenylalanine, tryptophan, and tyrosine.
- Optimum pH of 1.5 to 2. Pepsin denatures when the pH is more than 5.0.
Peptide cutter
PeptideCutter [references /
documentation]
predicts potential cleavage sites cleaved by proteases or chemicals in a given protein
sequence.
PeptideCutter returns the query sequence with the
possible cleavage sites mapped on it and /or a table of cleavage site positions.
