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Imperial College London/Notebook/2 October 2009
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(New page: {{Imperial/09/TemplateTop}} ==Testing== ===OD calibration 4th run 2nd Oct=== ====Protocol considerations==== *Cells were grown overnight in 10ml of M9 media *The cells were allowed to ...) |
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*OD of around 0.4 to 1.2 was made up, and subsequently diluted up to 10<sup>-4</sup> to 10<sup>-6</sup> | *OD of around 0.4 to 1.2 was made up, and subsequently diluted up to 10<sup>-4</sup> to 10<sup>-6</sup> | ||
*100ul of cells were inoculated into each plate | *100ul of cells were inoculated into each plate | ||
| - | *The OD of the overnight cells were measured again after 3 hours on ice to check | + | *The OD of the overnight cells were measured again after 3 hours on ice to check if cell number changes during dilution process |
====Results==== | ====Results==== | ||
Revision as of 19:09, 4 October 2009
Contents |
Testing
OD calibration 4th run 2nd Oct
Protocol considerations
- Cells were grown overnight in 10ml of M9 media
- The cells were allowed to grow until a high OD in the late morning, then placed in cold room
- OD of around 0.4 to 1.2 was made up, and subsequently diluted up to 10-4 to 10-6
- 100ul of cells were inoculated into each plate
- The OD of the overnight cells were measured again after 3 hours on ice to check if cell number changes during dilution process
Results
Waiting to be uploaded
Conclusions
Waiting to be uploaded
Suggestions/Improvements
Waiting to be uploaded
