Team:Imperial College London/Wetlab/Protocols/M1

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(Difference between revisions)
(→Protein Production (PP))
(→Protein Production (PP))
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| <b>PP2</b>: Lac characterisation
| <b>PP2</b>: Lac characterisation
| [[Team:Imperial_College_London/Wetlab/Protocols/IPTG-RFP |Lac characterisation]]
| [[Team:Imperial_College_London/Wetlab/Protocols/IPTG-RFP |Lac characterisation]]
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To investigate how RFP production induced by Lac promoter varies with IPTG <br>
+
|To investigate how RFP production induced by Lac promoter varies with IPTG <br>
| <b>PP3</b>: Cellulase
| <b>PP3</b>: Cellulase
| [[Team:Imperial_College_London/Wetlab/Protocols/Cellulase |Cellulase]]
| [[Team:Imperial_College_London/Wetlab/Protocols/Cellulase |Cellulase]]

Revision as of 21:48, 4 October 2009

Protein Production (PP)

Section Assay Overview and Aims
PP1: IPTG Toxicity IPTG Toxicity To investigate the effect of our IPTG inducer on growth of our cultures.
  • Measure growth rates at 28 degrees Celsius on minimal growth media so that subsequent testing timings etc. can be streamlined
  • Determine the effect of IPTG toxicity on growth w/o any protein production complications
PP2: Lac characterisation Lac characterisation To investigate how RFP production induced by Lac promoter varies with IPTG
PP3: Cellulase Cellulase Aims
PP4: PAH PAH Aims

Mr. Gene   Geneart   Clontech   Giant Microbes

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