Team:Imperial College London/M3/DamMethylation
From 2009.igem.org
(Difference between revisions)
(New page: {{Imperial/09/TemplateTop}} <html> <script type="text/javascript"> toggleToc(); </script> </html> {{Imperial/09/Tabs/M3}} ==Dam methylation== To protect against DNA destruction du...) |
|||
Line 13: | Line 13: | ||
<html><a href="https://2009.igem.org/Team:Imperial_College_London/Dam"><img style="vertical-align:bottom;" width=90px align="left" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Learnmore.png"></a></html><br><br> about the Dam methylation Defense Mechanism | <html><a href="https://2009.igem.org/Team:Imperial_College_London/Dam"><img style="vertical-align:bottom;" width=90px align="left" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Learnmore.png"></a></html><br><br> about the Dam methylation Defense Mechanism | ||
+ | |||
+ | <html><a href="https://2009.igem.org/Team:Imperial_College_London/Stomach"><img style="vertical-align:bottom;" width=90px align="left" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Learnmore.png"></a></html><br><br> About the ethical implications of live organisms. | ||
+ | |||
+ | |||
+ | <html><center><a href="https://2009.igem.org/Team:Imperial_College_London/M2"><img width=150px src="https://static.igem.org/mediawiki/2009/1/11/II09_M3ArrowLeft.png"></a><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control"><img width=150px src="https://static.igem.org/mediawiki/2009/b/bf/II09_M3_ArrowRight.png"></a></center> | ||
+ | </html> | ||
+ | |||
{{Imperial/09/Division}} | {{Imperial/09/Division}} |
Revision as of 20:38, 6 October 2009
- Overview
- Restriction Enzymes
- Dam Methylation
- Thermoinduction
Dam methylation
To protect against DNA destruction due to basal levels of restriction enzyme production, we have made use of the native E. coli Dam methylase protection system. This methylates DNA. Therefore, only high levels of restriction enzyme (ie. after thermal triggering) will cleave the DNA.
about the Dam methylation Defense Mechanism
About the ethical implications of live organisms.