From 2009.igem.org
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| + | *This morning I ran gels of my 2 colony PCR's as well as of my verification digest from Saturday. See gel photos below. |
| | + | *Because of these results, I also started to reconstruct R0040-I13502, doing a constriction digest with XbaI, EcorRI, an SpeI. I left this to digest overnight. |
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Revision as of 20:13, 9 October 2009
University of Calgary
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CAROL
Descriptive Title of What You’re Doing
WIKI CODING HERE
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CHINMOYEE
Weekly Modelling Meeting
This meeting was a really long one ( 3 hours ) . A comparison between between Membrane computing values and Matlab interface values were made. The units of the k values was discussed. The size of the Kdegradation of GFP was confirmed through calculation.
There was a question about what infinity meant when placing initial values : Should the Constant value option in Matlab be used or Should the infinity mean a large value in comparison to to the other values ie 100 units etc.
Can Membrane computing interface produce similar results as the Matlab model when only one cell is simulated.
The Model Summary( version 4.0) was presented again so that intial values and parameter values could be passed between the two teams.
A new problem in the Differential equation model was detected. The system doesn't seem to have a saturation point for AI2 . The GFP level in the graph always decreases faster with more AI2 is added to the system .
Possible reasons for this :
A few values of parameters are not in the correct order.
That is the nature of the model . It can't doesn't have a saturation point .
A new meeting to resovle the possible faulty value for the parameters was planned for Thursday at 4pm.
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EMILY
*This morning I ran gels of my 2 colony PCR's as well as of my verification digest from Saturday. See gel photos below.
*Because of these results, I also started to reconstruct R0040-I13502, doing a constriction digest with XbaI, EcorRI, an SpeI. I left this to digest overnight.
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FAHD
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IMAN
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JAMIE
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JEREMY
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KATIE
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KEVIN
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MANDY
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PATRICK
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PRIMA
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STEFAN
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