Team:UNIPV-Pavia/Methods Materials/Measurement

From 2009.igem.org

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Revision as of 22:38, 12 October 2009

Measurements

A preliminar study was done on the instrument TECAN Infinite F200, a multifunctional microplate filter-based reader described in Instruments, to understand the behaviour of the measurements of absorbance and fluorescence intensity in different situations.

The results of the experiments, grouped by argoments, are described here, if you need more informations about a single experiment, you can find the detailed protocol at [http://aimed11.unipv.it/iGEM2009 Download Protocols].

Studies:

ABSORBANCE:

FLUORESCENCE:

EVAPORATION:

The "frame effect" on the plate


	CONCLUSIONS: EXPERIMENTAL SET UP AND MEASUREMENT METHODS

	• In long experiments do not use the well on the frame, but fill them with water or growth medium to maintain the temperature homogenous in the plate; • in long experiments cover the plate with the lid to limit the evaporation; • use the O.D. measurements to quantify bacterial growth, regardless of the volume in the well; • do not dispense water if you perform fluorescence measurements; • work with volumes not larger than 200 μl and prefer the M9 medium, if you perform fluorescence measurements; • in long experiments, if you perform fluorescence measurements on fluorescent coltures, always subtract the blank value, to correct the descending evolution of the fluorescence during the incubation.

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 • work with volumes not larger than 200 μl and prefer the M9 medium, if you perform fluorescence measurements;<br><br>
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+• in long experiments, if you perform fluorescence measurements on fluorescent coltures, always subtract the blank value, to correct the descending evolution of the fluorescence during the incubation.
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