Team:ArtScienceBangalore/Notebook

From 2009.igem.org

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=== '''May 15th''' ===
 
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This was the day we officially started working on our iGEM 2009 project.
 
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This week was solely reserved for introducing us to the unfamiliar territory of what we were getting into.
 
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We discussed two 'Claire Pentacost' Readings-
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== About ==
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We consider ourselves amateurs/novices within the context of the IGEM competition. Our endeavor as “outsiders” is to bring our training in the arts and design to synthetic biology. Over this summer, we learnt the tools and techniques of synthetic biology and developed a piece of life which reflects our concerns, namely, the cultural, ethical and aesthetic implications of Synthetic Biology. Using a DIY approach and getting our hands “wet” was a critical element in the learning process. This approach allowed us to engage in constructive discussions with scientists working in the field. Our construct synthesizes Geosmin, an enzyme normally produced by cyanobacteria and actinobacteria. The biosynthesis of geosmin from farnesyl diphosphate is catalyzed by a single enzyme germacradienol/germacrene D synthase. E. coli, does not bear a gene that codes for germacradienol/germacrene D synthase. We have expressed this gene in different strains of  E. coli, under control of the pTrc  promoter present in the pTrc99a vector. Geosmin is responsible for producing the earthy smell that occurs in the air when rain falls after a dry spell of weather.
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'''Beyond Face:'''
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== People ==
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[http://www.clairepentecost.org/beyond_face.htm Beyond Face ]
 
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'''Critical Inventory of BioArt:'''
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[[User:navadrian.cinthus|Akash Hirosh]]
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[http://www.clairepentecost.org/lab%20of%20symbolic_text.htm Critical Inventory of BioArt ].
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[[User:iamnosuperman|Dhruv Nawani]]
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'''The gist of these readings:'''
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[[Nikhil Patil]]
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Artists work with the symbolic.The artist's consent to work and learn in public is important.
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[[Upasana Simha]]
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We discussed the political and cultural implications of Scientific Authority.
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[[User:Sem2490|Sandeep Mathew]]
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We also looked at Tuur Van Balen's :
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'''The Urban Geography Project:'''
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[[Sanya Rai Gupta]]
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[http://www.tuurvanbalen.com/projects/urbanbiogeography Urban Geography project]
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[[Avni Sethi]]
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Most of the ideas today, dealt with the use of bacteria as:  
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[[User:NehaBhat|Neha Bhat]]
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a) A sensor or Reactor - (to inputs,emotions,light etc)
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[[User:Gautamf472|Gautam Vishwanath]]
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b) A Producer (of energy, proteins etc)
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[[Krupakar Dhinakaran]]
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c) A Material
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==Ideas==
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-Is there any way in which we can look at bacteria from a purely non-symbiotic / non-anthropomorphic viewpoint?
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[[File:Whiteboard.jpg|820px|none|Click for a closer look]]
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-Can we use our technological "progress" to give a non-selfish gift back to our ecological siblings?
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Major Outcomes -
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-Can we replace financial transactions with Bacteria?
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* [[Ideas for Bacteria.]]
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=== '''May 16th''' ===
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* Facilitate a group of children to design their 'perfect creature' through visual/tactile media and then draw from these experiences and ideas to build another structure.
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We asked ourselves to think of our own theoritical bacteria and represent it on paper.
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*Basic information on micro biology-synthetic biology becomes accessible in the forms of brochures/charts/posters in the team's work space.
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Here are some creatures we created using techno-scientific jargon and aesthetics:
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== Workshop ==
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<gallery>
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<img src ="http://hackteria.org/wiki/images/0/03/Non-standard_registry_of_names.jpg"></img>|Upasana's 'Non Standard Registry of Names'
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File:http://hackteria.org/wiki/images/2/22/Upasana.jpg|Upasana's Ideas
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File:http://hackteria.org/wiki/images/c/cd/Bacteria_own.jpg|Neha's 'Materialistica Destructica'
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File:http://hackteria.org/wiki/images/d/db/DOC160509-16052009101732_Page_07.jpg|Neha's Graph
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File:Page_1.jpg| Avni's ''Actinodopaine''
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File:Description_of_the_Amebitoni_Bacteria.png|Sandeep's 'Amebitoni Bacteria'
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File:2.jpg|Krupakar's 'Immuno Bacteria'
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File:Sanya1.jpg|Sanya's 'Plastico Collecticus'
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File:bactiprint.jpg|Akash's 'ID bacteria'
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File:bacteria.jpg|Nikhil's ideas
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</gallery>
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'''Today's Readings:'''
 
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'''Speculative Fabulations for Technoculture's Generation''':
 
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[http://www.patriciapiccinini.net/essay.php?id=30 Speculative Fabulations for Technoculture's Generation]
 
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-The article is primarily a review of the Australian artist Patricia Piccinini's work and a recapitulation of Haraway's philosophies .
 
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-One of the things enduring about the reading was her appeal to "love" our creations, not in a techno- phillic sense but in a more nurturing and caring way.
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{| border="0" cellspacing="0" cellpadding="4" align="center"
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![[Week One]]
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![[Week Two]]
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![[Week Three]]
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![[Week Four]]
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|-
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![[Week Five]]
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![[Week Six]]
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![[Week Seven]]
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![[Week Eight]]
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|}
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=== '''May 17th''' ===
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<br/>
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We looked up some hybrid creatures from mythology to expand the scope of our imagination.
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=== Other ===
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'''Hybrid Creatures From Mythology:'''
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http://www.historyforkids.org/scienceforkids/biology/cells/doing/dna.htm
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[[Gallery]]
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'''Notes From NCBS:'''
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It is helpful to have an idea of how the other teams are progressing. So, if anyone comes across interesting iGEM team wikis/sites - please put up the links here.
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*[[The May 17th Notebook]]
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http://igem.uwaterloo.ca/The_UW_iGEM_Team
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=== '''May 19th''' ===
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The IBB Pune Team's wiki
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[https://2009.igem.org/Team:IBB_Pune#]
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We spent the day in NCBS picking up some standard biological techniques such as 'Gel Electrophoresis' and looking at some of the microscopy equipment at NCBS.
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[https://2008.igem.org/Team:NYMU-Taipei NYMU-Taipei's 2008 Wiki]
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*[[Mukund's Brief Overview on Synthetic Biology Basics]]
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== Readings ==
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*[[Gel Electrophorosis]]
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'''[[Art and Politics]]'''
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*([[Talk:ArtScience_IGEM_team|Click here to discuss]])
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'''[[Art]]'''
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*[[Streaking and Spreading to obtain bacterial colonies]]  
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'''[[Science]]'''
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*[[Imaging]]
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'''[[Ethics]]'''
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*[[Discussion over Lunch]]
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'''[[Design & Technology]]'''
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'''[[Synthetic Biology]]'''
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[[Some Images Of Our Day ]]
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'''[[General Design Links]]'''
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Non-categorised images [[here]]
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=== '''May 21st''' ===
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We put down all the information that we had about learnt about geosmin. We then put down the various paths we could take in order to produce the results we wanted.
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This gave us a dummy model of how we could achieve the final result.
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We divided ourselves into two groups and came up with '''two distinct approaches''' of representing the same model.This exercise cleared certain doubts we had, but also raised a lot of questions.
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<gallery>
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File:geosmin_synthesis.jpg|The Scientific Representation
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File:Rain_scenario_1.jpg
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File:RAin_scenario_2.jpg
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File:rain_scenario_3.jpg
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File:Bactothing2.png|The Artistic Representation
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</gallery>
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[[First Prototype of the Bacteria]]
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Revision as of 10:18, 14 October 2009

Home The Team Aproach The Project Outreach Links Gallery


Contents

About

We consider ourselves amateurs/novices within the context of the IGEM competition. Our endeavor as “outsiders” is to bring our training in the arts and design to synthetic biology. Over this summer, we learnt the tools and techniques of synthetic biology and developed a piece of life which reflects our concerns, namely, the cultural, ethical and aesthetic implications of Synthetic Biology. Using a DIY approach and getting our hands “wet” was a critical element in the learning process. This approach allowed us to engage in constructive discussions with scientists working in the field. Our construct synthesizes Geosmin, an enzyme normally produced by cyanobacteria and actinobacteria. The biosynthesis of geosmin from farnesyl diphosphate is catalyzed by a single enzyme germacradienol/germacrene D synthase. E. coli, does not bear a gene that codes for germacradienol/germacrene D synthase. We have expressed this gene in different strains of E. coli, under control of the pTrc promoter present in the pTrc99a vector. Geosmin is responsible for producing the earthy smell that occurs in the air when rain falls after a dry spell of weather.

People

Akash Hirosh

Dhruv Nawani

Nikhil Patil

Upasana Simha

Sandeep Mathew

Sanya Rai Gupta

Avni Sethi

Neha Bhat

Gautam Vishwanath

Krupakar Dhinakaran

Ideas

Click for a closer look

Major Outcomes -

  • Facilitate a group of children to design their 'perfect creature' through visual/tactile media and then draw from these experiences and ideas to build another structure.
  • Basic information on micro biology-synthetic biology becomes accessible in the forms of brochures/charts/posters in the team's work space.

Workshop

Week One Week Two Week Three Week Four
Week Five Week Six Week Seven Week Eight


Other

http://www.historyforkids.org/scienceforkids/biology/cells/doing/dna.htm

It is helpful to have an idea of how the other teams are progressing. So, if anyone comes across interesting iGEM team wikis/sites - please put up the links here.

http://igem.uwaterloo.ca/The_UW_iGEM_Team

The IBB Pune Team's wiki [1]

NYMU-Taipei's 2008 Wiki

Readings

Art and Politics

Art

Science

Ethics

Design & Technology

Synthetic Biology

General Design Links

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