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Team:Imperial College London/M1
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<i>The E.ncapsulator</i> has been designed to produce and deliver polypeptides (amino acid polymers) to the intestine. <b>Module 1</b> encompasses the polypeptide production phase. During this period, our polypeptide of interest is synthesised at a rate sufficient to faciliate its accumulation inside the cell. | <i>The E.ncapsulator</i> has been designed to produce and deliver polypeptides (amino acid polymers) to the intestine. <b>Module 1</b> encompasses the polypeptide production phase. During this period, our polypeptide of interest is synthesised at a rate sufficient to faciliate its accumulation inside the cell. | ||
| - | To demonstrate <i>The E.ncapsulator</i>'s versatility, we have chosen to showcase it with both enzymes and peptides. These two classes of polypeptide have very different properties that we have considered and catered for in The <i>E.ncapsulator</i>'s design. | + | To demonstrate <b><i>The E.ncapsulator</i></b>'s versatility, we have chosen to showcase it with both enzymes and peptides. These two classes of polypeptide have very different properties that we have considered and catered for in The <i>E.ncapsulator</i>'s design. |
| - | <br> | + | <br><html><a href="https://2009.igem.org/Team:Imperial_College_London/M1_Detail"><img style="vertical-align:bottom;" width=50px align="left" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Learnmore.png"></a></html> <b><i>About the difference between enzymes and peptides.</i></b><br>< |
| - | + | We have chosen two enzymes to showcase <b><i>The E.ncapsulator</i></b>'s protein production module.<br> | |
| - | <html><a href="https://2009.igem.org/Team:Imperial_College_London/M1_Detail"><img style="vertical-align:bottom;" width=50px align="left" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Learnmore.png"></a></html> <b><i>About the difference between enzymes and peptides.</i></b><br><br> | + | These are:<br> |
| - | + | *Cellulase - an enzyme that breaks down the tough fibrous molecule cellulose into cellobiose. | |
| + | *Phenylalanine | ||
<b>Module 1</b> is chemically induced by the addition of IPTG. After sufficient amount of protein of interest has been produced, autoinduction occurs and encapsulation will start. | <b>Module 1</b> is chemically induced by the addition of IPTG. After sufficient amount of protein of interest has been produced, autoinduction occurs and encapsulation will start. | ||
Revision as of 12:18, 15 October 2009
Contents |
Module 1: Protein Production
Overview
The E.ncapsulator has been designed to produce and deliver polypeptides (amino acid polymers) to the intestine. Module 1 encompasses the polypeptide production phase. During this period, our polypeptide of interest is synthesised at a rate sufficient to faciliate its accumulation inside the cell.
To demonstrate The E.ncapsulator's versatility, we have chosen to showcase it with both enzymes and peptides. These two classes of polypeptide have very different properties that we have considered and catered for in The E.ncapsulator's design.
About the difference between enzymes and peptides.
<
We have chosen two enzymes to showcase The E.ncapsulator's protein production module.
These are:
- Cellulase - an enzyme that breaks down the tough fibrous molecule cellulose into cellobiose.
- Phenylalanine
Module 1 is chemically induced by the addition of IPTG. After sufficient amount of protein of interest has been produced, autoinduction occurs and encapsulation will start.
Project Tour
