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Team:Imperial College London/Temporal Control
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| - | <html><center><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Chemical_Induction"><img style="vertical-align:bottom;" width=" | + | <html><center><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Chemical_Induction"><img style="vertical-align:bottom;" width="15%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_chemicalinduction.png"></a><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Autoinduction"><img style="vertical-align:bottom;" width="15%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Drylabmainimage1.png"></a><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Thermoinduction "><img style="vertical-align:bottom;" width="15%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Thermoinduction1.png"></a><a href="https://2009.igem.org/Team:Imperial_College_London/Drylab"><img style="vertical-align:bottom;" width="15%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Wetlabmainimage9.png"></a><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Modelling"><img style="vertical-align:bottom;" width="15%" |
| - | src="http://i691.photobucket.com/albums/vv271/dk806/II09_Wetlabmainimage9.png"></a><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Modelling"><img style="vertical-align:bottom;" width=" | + | src="http://i691.photobucket.com/albums/vv271/dk806/II09_Wetlabmainimage9.png"></a><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Modelling"><img style="vertical-align:bottom;" width="15%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Drylabmainimage6.png"></a></center></html> |
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| - | <td width=" | + | <td width="15%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Chemical_Induction"><b>Chemical Induction</b></a></center></td> |
| - | <td width=" | + | <td width="15%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Autoinduction"><b>Autoinduction</b></a></center></td> |
| - | <td width=" | + | <td width="15%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Thermoinduction"><b>Thermoinduction</b></a></center></td> |
| - | <td width=" | + | <td width="15%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/Drylab"><b>Wet Lab</b></a></center></td> |
| - | <td width=" | + | <td width="15%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/Wetlab/Results#Temporal_Control/Modelling"><b>Modelling</b></a></center></td> |
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| - | <td width=" | + | <td width="15%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Chemical_Induction"><b>Testing construct and graph</b></a></center></td> |
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Revision as of 18:31, 15 October 2009
Contents |
Temporal Control
In our project there are 3 forms of temporal control that have been implemented.
- Chemical induction: Triggers the production of the polypeptide of interest using IPTG. Effectively 'kicks off' the system once the cell density is high enough.
- Autoinduction: Represses encapsulation when glucose levels are high, and kick starts it once glucose is used up. This allows a sufficient amount of protein production to have taken place before the cell focuses its resources on encapsulation.
- Thermoinduction: Triggers genome deletion when the temperature is increased. Thermoinduction was necessary, as chemical induction may be blocked by the presence of the capsule (that inhibits diffusion).
Testing Construct
This testing construct was used to test the inducible promoters using flourescent proteins as output reporters.
The timeline shows the sequence of occurrence of these events:
Explainations for temporal overview
Please click on the table for a clearer view.
Project Tour
For more details of the temporal control of the system, see the tabs below.
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Temporal Control Contents
