"
HKU-HKBU/5 August 2009
From 2009.igem.org
(Difference between revisions)
Paultse (Talk | contribs)
(New page: 2009-8-5 Co-transformation =====12:50 pre-culture MG3 in rotator (32℃) for 3h===== =====15:50 take bacteria out from rotator===== =====16:10 sub-culture MG3 in warm room (37℃) for 1...)
Newer edit →
(New page: 2009-8-5 Co-transformation =====12:50 pre-culture MG3 in rotator (32℃) for 3h===== =====15:50 take bacteria out from rotator===== =====16:10 sub-culture MG3 in warm room (37℃) for 1...)
Newer edit →
Revision as of 14:48, 16 October 2009
2009-8-5 Co-transformation
Contents |
12:50 pre-culture MG3 in rotator (32℃) for 3h
15:50 take bacteria out from rotator
16:10 sub-culture MG3 in warm room (37℃) for 1.5h
17:40 take bacteria out from warm room
Competent Cell Preparation Electro-transformation ① A+ & C+ ② A+ & C+ ③ A+ ④ C+ After transformation, recovering (1h) and spreading plates, put these plates into warm room (37℃) for overnight Suppose: ① A+ & C+ →grow ② A+ & C+ →grow ③ A+ →grow ④ C+ →grow
