Team:British Columbia

From 2009.igem.org

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|Formed in March 2009, this iGEM team is the first to enter from UBC, and hopes to do its alma mater proud.
 
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==The Bacterial Traffic Light==
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We have selected two project tracks:
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The goal of our project is to construct an ''Eschericia coli'' bacterial "traffic light", that responds to increasing concentration levels of a given input (in our case, arabinose) with green, yellow, and red fluorescent outputs. Our project is unique because it is an analog biosensor, capable of responding to a continuous variable signal, rather than a binary biosensor that only signals on/off.
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* To manipulate the sensitivity of a whole-cell biosensor
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* To implement a logic gate-based decision system using said biosensor
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The Traffic Light is composed of three distinct subparts:
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The general scheme is to prototype the manipulations using an arabinose sensor coupled to a GFP reporter.
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* The [[Team:British_Columbia/pBAD|pBAD promoter family]]
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* The [[Team:British_Columbia/LockandKey|lock and key riboregulation system]]
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[[Image:UBC2009-kayaking.jpg|centre|frame|Our Team]]
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* The [[Team:British_Columbia/Jammer|Jammer]]
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For a summary of our actual results, see [[Team:British_Columbia/Judging_Form|here]].

Revision as of 17:55, 17 October 2009

IGEMFinalGoldDNA-logo.jpg Home The Team The Project Parts Submitted to the Registry Sponsor Us! General Lab Notebook Bibliography
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IGEMFinalGoldDNA-logo.jpg

The Bacterial Traffic Light

The goal of our project is to construct an Eschericia coli bacterial "traffic light", that responds to increasing concentration levels of a given input (in our case, arabinose) with green, yellow, and red fluorescent outputs. Our project is unique because it is an analog biosensor, capable of responding to a continuous variable signal, rather than a binary biosensor that only signals on/off.

The Traffic Light is composed of three distinct subparts:

For a summary of our actual results, see here.