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Lab July 27 2009
From 2009.igem.org
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(New page: Checked plates - growth on all 4 plates new DH5alpha plates look contaminated. Staph? Not sure. old competent cells look OK. Should we just go back to our old cell line? It seems t...)
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(New page: Checked plates - growth on all 4 plates new DH5alpha plates look contaminated. Staph? Not sure. old competent cells look OK. Should we just go back to our old cell line? It seems t...)
Newer edit →
Revision as of 21:25, 17 October 2009
Checked plates - growth on all 4 plates
new DH5alpha plates look contaminated. Staph? Not sure.
old competent cells look OK. Should we just go back to our old cell line? It seems the issue was a plasmid dilution issue rather than a competent cells issue.
re-plating with the following plasmid dilutions:
currently have 23ng/ul, to get to 10ng/ul mix 4.35ul plasmid and 5.65 ul dH2O
1 ng = 1ul of previous mix in 9ul of dH2O
.01 ng = 1ul of previous mix in 9ul of dH2O
after mixing each plasmid concentration, follow the Transformation Protocol.
