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Lab Sept 01 2009
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Jasoncummer (Talk | contribs) (New page: Kyliah says: I'll come in today for the afternoon. Did the stabs ever show up? If not, we might be able to substitute something out for the arabinose operon. 11.00am Well nothing grew...) |
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K235010=J06504(Am)+B0015(AmpKan)+P1010Cm - This one should have worked, though. | K235010=J06504(Am)+B0015(AmpKan)+P1010Cm - This one should have worked, though. | ||
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Latest revision as of 22:16, 17 October 2009
Kyliah says: I'll come in today for the afternoon. Did the stabs ever show up? If not, we might be able to substitute something out for the arabinose operon.
11.00am
Well nothing grew last night so we have some problems. There is one tiny suspicious colony on a P1010 kan plate but I can't imagine it will be correct.
On the plus side, between Dr. Upton and Dr. Nano we should have bench space, along with 4C, -20C and -80C space, so everything we need for the fall!
I'm going to start by running a gel for the parts used yesterday: J23032(Amp) downstream, J23102(Amp) upstream, B0015(AmpKan) downstream, J06504(Amp) upstream, K235000(Cm) downstream
Gel 1 September 1
Started at 2.30pm
1 Kb Ladder J23032 Down
J23102
Up B0015 Down J06504 Up K235000 Down P K081005 P K098988
No Band Band Band No No Band
sep 1 gel 1.tif
K235009=J23102(Amp)+J23032(Amp)+P1010Cm - No wonder this assembly didn't work, one of the ligation parts seems to be absent
K235010=J06504(Am)+B0015(AmpKan)+P1010Cm - This one should have worked, though.
Back to LAB NOTEBOOK
