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Team:Wisconsin-Madison/Restriction Digestion
From 2009.igem.org
(Difference between revisions)
(New page: {{Wisconsin-Madison Template}} {{Wisconsin-Madison Note Template}} <br> {| class="wikitable" border="0" align="center" width=790px |style="text-align:left" style="vertical-| <center> ''...) |
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| - | Use smallest micro cuvettes and mix: | + | Use smallest micro cuvettes and mix (total volume 18 μL): |
| + | |||
1.8 μL Buffer (10x) | 1.8 μL Buffer (10x) | ||
| + | |||
0.18 μL BSA +3 (100x) | 0.18 μL BSA +3 (100x) | ||
| + | |||
12 μL MiliQ H20 | 12 μL MiliQ H20 | ||
| + | |||
3 μL DNA | 3 μL DNA | ||
| + | |||
1 μL Enzyme (100x) | 1 μL Enzyme (100x) | ||
| - | Incubate in water bath at 37 C for 2 hours | + | |
| + | Incubate in water bath at 37 C for 2 hours: | ||
| + | |||
3.6 μL Dye (6x) | 3.6 μL Dye (6x) | ||
<center>[[Team:Wisconsin-Madison/Notebook_Protocols|'''Back to Protocols''']]</center> | <center>[[Team:Wisconsin-Madison/Notebook_Protocols|'''Back to Protocols''']]</center> | ||
Revision as of 22:17, 17 October 2009
Restriction Enzyme Mapping Use smallest micro cuvettes and mix (total volume 18 μL): 1.8 μL Buffer (10x) 0.18 μL BSA +3 (100x) 12 μL MiliQ H20 3 μL DNA 1 μL Enzyme (100x) Incubate in water bath at 37 C for 2 hours: 3.6 μL Dye (6x)
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