Team:UNIPV-Pavia/Methods Materials/Glycerol stock

From 2009.igem.org

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''(estimated time: 15 min + freezing time)''
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''(estimated time: 15 min + freezing time)''''Italic text''
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Revision as of 15:16, 22 June 2009

EthanolPVanimation.gif

Protocols


=Gycerol stock=
(estimated time: 15 min + freezing time)'Italic text

Materials needed:

  • sample of overnight incubated bacteria
  • 80% glycerol


  • Mix 750 ul from the 5 ml sample of overnight incubated bacteria with 250 ul of 80% glycerol
  • Leave at -20°C for one day
  • Move to -80°C the day after
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