Team:UNIPV-Pavia/Methods Materials/XGal

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(New page: {{UNIPV-Pavia/Protocols}} <br> == '''X-Gal staining protocol for beta galactosidase (blue/white screening)''' == ''(Estimated time: 20 min)'' <br> <br> '''Materials needed:''' *'''40 mg/m...)
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== '''X-Gal staining protocol for beta galactosidase (blue/white screening)''' ==  
== '''X-Gal staining protocol for beta galactosidase (blue/white screening)''' ==  

Revision as of 17:20, 22 June 2009

EthanolPVanimation.gif



Protocols


X-Gal staining protocol for beta galactosidase (blue/white screening)

(Estimated time: 20 min)

Materials needed:

  • 40 mg/ml X-gal in DMF
  • SOC medium
  • pre-warmed LB agar plate
  • face mask


  • The principle is that X-Gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside) turns blue when reacts with beta-galactosidase.
  • Mix 20 ul X-Gal 20 mg/ml and 80 ul SOC and spread on LB+amp plates (X-Gal and DMF are toxic, use face-mask for your safety!!! X-Gal is light-sensitive, remember to keep it in the dark, when possible)
  • Incubate with 200 ul bacteria
  • Result: blue colonies express LacZ, while white colonies don't.
Retrieved from "http://2009.igem.org/Team:UNIPV-Pavia/Methods_Materials/XGal"