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Team:Imperial College London/Autoinduction
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<b><i>The E.ncapsulatior</i></b> has been designed to be able to withstand the rigours of stomach acid and freeze drying. This is achieved by the synthesis of the exopolysaccharide colanic acid and the cryoprotectant trehalose. | <b><i>The E.ncapsulatior</i></b> has been designed to be able to withstand the rigours of stomach acid and freeze drying. This is achieved by the synthesis of the exopolysaccharide colanic acid and the cryoprotectant trehalose. | ||
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Revision as of 15:47, 18 October 2009
Module Integration - Autoinduction
The E.ncapsulatior has been designed to be able to withstand the rigours of stomach acid and freeze drying. This is achieved by the synthesis of the exopolysaccharide colanic acid and the cryoprotectant trehalose.
Initiation:
Module 2 is triggered by a rise in levels of cyclic Adenosine Mono Phosphate (cAMP). This is because cAMP facilitates the dimerisation of the transcription factor CRP which induces transcription downstream of the PcstA promoter. The rise in cAMP is correlated with a fall in levels of the primary carbon source (glucose). Thus, by controlling the initial amount of glucose present in the system, we achieve a timer function for the start of Module 2.
About Module 2 temporal control.
