Revision as of 18:49, 18 October 2009

Contents

1 Regulatory Elements
- 2.1 Promoter
2 Alcohol Production
3 Pyruvate Level Increasing Approaches
- 3.1 Pir Approach
- 3.2 Antisense RNA Approach

Parts

Here we list all the BioBricks we designed, constructed and used during the project.

Regulatory Elements
Promoter
BBa_K273019	Regulatory	pPetE	429
Alcohol Production
Butanol Parts, Intermediates and Devices
BBa_K273006	Coding	a-ketoisovalerate decarboxylase (kivd) codon optimized	1650
BBa_K273008	Intermediate	RBS - a-ketoisovalerate decarboxylase (kivd) codon optimized	1668
BBa_K273005	Coding	Alcohol Dehydrogenase 2 (Yadh2)	1050
BBa_K273007	Intermediate	RBS - Alcohol Dehydrogenase 2 (Yadh2)	1068
BBa_K273009	Intermediate	RBS - adh2(Y) - Terminator	1205
BBa_K273012	Intermediate	RBS - kivd - RBS - adh2 - Ter	2881
BBa_K273011	Composite	PLlac - RBS - kivd - RBS - adh2(Y) - Ter	2944
BBa_K273012	Composite	pPetE - RBS - kivd - RBS - adh2(Y) - Ter	3318
Ethanol Parts and Devices
BBa_K273000	Coding	Pyruvate decarboxylase (pdc)	1710
BBa_K273001	Coding	Alcohol dehydrogenase (Zadh2)	1155
BBa_K273004	Intermediate	RBS - Pyruvate decarboxylase (pdc)	1728
BBa_K273017	Intermediate	RBS - Alcohol dehydrogenase (Zadh2)	1173
BBa_K273018	Intermediate	RBS - adh2(Z) - Ter	1310
BBa_K273013	Intermediate	RBS - pdc - RBS -adh2(Z) - Ter	3046
BBa_K273056	Composite	PLlac - RBS - pdc - RBS - adh2(Z) - Ter	3109
BBa_K273057	Composite	pPetE - RBS - pdc - RBS - adh2(Z) - Ter	3483
Ethanol - S. cerevisae
BBa_K273014	Intermediate	RBS + pdc + RBS + adh2(Y) + Ter	2941
BBa_K273015	Composite	PLlac - RBS - pdc - RBS - adh2(Y) - Ter	3004
BBa_K273016	Composite	pPetE - RBS - pdc - RBS - adh2(Y) - Ter	3378
Pyruvate Level Increasing Approaches
Pir Approach
BBa_K273002	Coding	Pirin like protein (pirA)	702
BBa_K273003	Coding	Pirin like protein (pirB)	240
Antisense RNA Approach
BBa_K273020	RNA	E1A1	20
BBa_K273021	RNA	E1A2	30
BBa_K273022	RNA	E1A3	50
BBa_K273023	RNA	E1A4	30
BBa_K273024	RNA	E1B1	30
BBa_K273025	RNA	E1B2	30
BBa_K273026	RNA	E1B3	20
BBa_K273027	RNA	E1B4	37
BBa_K273028	RNA	E21	30
BBa_K273029	RNA	E22	30
BBa_K273030	RNA	E23	20
BBa_K273031	RNA	E24	50
BBa_K273032	Intermediate	E1A1 - Ter	157
BBa_K273033	Intermediate	E1A2 - Ter	167
BBa_K273034	Intermediate	E1A3 - Ter	187
BBa_K273035	Intermediate	E1A4 - Ter	167
BBa_K273036	Intermediate	E1B1 - Ter	167
BBa_K273037	Intermediate	E1B2 - Ter	167
BBa_K273038	Intermediate	E1B3 - Ter	157
BBa_K273039	Intermediate	E1B4 - Ter	174
BBa_K273040	Intermediate	E21 - Ter	167
BBa_K273041	Intermediate	E22 - Ter	167
BBa_K273042	Intermediate	E23 - Ter	157
BBa_K273043	Intermediate	E24 - Ter	187
BBa_K273044	Composite	pPetE - E1A1 - Ter	594
BBa_K273045	Composite	pPetE - E1A2 - Ter	604
BBa_K273046	Composite	pPetE - E1A3 - Ter	624
BBa_K273047	Composite	pPetE - E1A4 - Ter	604
BBa_K273048	Composite	pPetE - E1B1 - Ter	604
BBa_K273049	Composite	pPetE - E1B2 - Ter	604
BBa_K273050	Composite	pPetE - E1B3 - Ter	594
BBa_K273051	Composite	pPetE - E1B4 - Ter	611
BBa_K273052	Composite	pPetE - E21 - Ter	604
BBa_K273053	Composite	pPetE - E22 - Ter	604
BBa_K273054	Composite	pPetE - E23 - Ter	594
BBa_K273055	Composite	pPetE - E24 - Ter	624

@@ Line 14: / Line 14: @@
      <li class="toclevel-2"><a href="#but"><span class="tocnumber">2.1</span> <span class="toctext">Butanol Parts, Intermediates and Devices</span></a></li>
      <li class="toclevel-2"><a href="#eth1"><span class="tocnumber">2.2</span> <span class="toctext">Ethanol Parts, Intermediates and Devices</span></a></li>
-     <li class="toclevel-2"><a href="#eth2"><span class="tocnumber">2.3</span> <span class="toctext">Ethanol Parts, Intermediates and Devices with adh2 from S. cerevisae</span></a></li>
+     <li class="toclevel-2"><a href="#eth2"><span class="tocnumber">2.3</span> <span class="toctext">Ethanol Parts - S. cerevisae</span></a></li>
     </ul>
      </li>
@@ Line 34: / Line 34: @@
    <TABLE WIDTH="100%" BORDER="0" CELLPADDING="1">
    <TR>
-     <TD>
+     <TD colspan="4">
-      <a class="ah2" name="reg">Regulatory Elements</a><br>
+      <a class="ah1" name="reg">Regulatory Elements</a><br><br>
      </TD>
    </TR>
      <TR>
-     <TD>
+     <TD colspan="4">
       <a class="ah3" name="pro">Promoter</a><br>
      </TD>
@@ Line 60: / Line 60: @@
    </TR>
      <TR>
-     <TD>
+     <TD colspan="4">
-      <a class="ah1" name="prod">Alcohol Production</a><br>
+      <br><a class="ah1" name="prod">Alcohol Production</a><br><br>
      </TD>
    </TR>
    <TR>
-     <TD>
+     <TD colspan="4">
-      <a class="ah1" name="but">Butanol Parts, Intermediates and Devices</h2></a><br>
+      <a class="ah3" name="but">Butanol Parts, Intermediates and Devices</a><br>
      </TD>
    </TR>
@@ Line 182: / Line 182: @@
      </TD>
      <TD>
       Composite
      </TD>
@@ Line 192: / Line 191: @@
      </TD>
      <TD>
      </TD>
    </TR>
    <TR>
    </TR>
    <TR>
-     <TD>
+     <TD colspan="4">
-      <a class="ah3" name="eth1">Ethanol Parts and Devices</h2></a>
+      <br><a class="ah3" name="eth1">Ethanol Parts and Devices</a><br>
      </TD>
    </TR>
@@ Line 330: / Line 327: @@
    </TR>
    <TR>
-     <TD>
+     <TD colspan="4">
-      <a class="ah3" name="eth2">Ethanol - <i>S. cerevisae</i></a>
+      <br><a class="ah3" name="eth2">Ethanol - <i>S. cerevisae</i></a><br>
      </TD>
    </TR>
@@ Line 383: / Line 380: @@
    </TR>
    <TR>
-     <TD>
+     <TD colspan="4">
-      <a class="ah1" name="pyr">Pyruvate Level Increasing Approaches</a>
+      <br><a class="ah1" name="pyr">Pyruvate Level Increasing Approaches</a><br><br>
      </TD>
    </TR>
    <TR>
-     <TD>
+     <TD colspan="4">
-      <a class="ah3" name="pir">Pir Approach</a>
+      <a class="ah3" name="pir">Pir Approach</a><br>
      </TD>
    </TR>
@@ Line 426: / Line 423: @@
    </TR>
    <TR>
+     <TD colspan="4">
-     <TD>
+      <br><a class="ah3" name="rna">Antisense RNA Approach</a><br>
-      <a class="ah3" name="rna">Antisense RNA Approach</a>
      </TD>
    </TR>

Team:Uppsala-Sweden/Parts

From 2009.igem.org

Revision as of 18:49, 18 October 2009

Parts