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Team:UNIPV-Pavia/Methods Materials/Glycerol stock
From 2009.igem.org
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*'''80% glycerol''' | *'''80% glycerol''' | ||
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Revision as of 23:04, 23 June 2009
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Gycerol stocks
(estimated time: 15 min + freezing time)
Materials needed:
- Sample of overnight incubated bacteria
- 80% glycerol
- Mix 750 ul from the 5 ml sample of overnight incubated bacteria with 250 ul of 80% glycerol
- Leave at -20°C for one day
- Move to -80°C the day after
