Team:KULeuven/29 September 2009

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Revision as of 11:36, 19 October 2009

Project progress

Progress of parts

[edit] Blue Light Receptor

we redid the experiment from 24/09 but only grew cultures at 25°C. we did split up the cultures into two groups, 16°c and 25°C and each group had some cultures that were lit and some dark.

[edit] Vanillin Production

  • Nanodrop of the six cultures of PAMSCOEFT
part concentration 260/280 260/230
PAMSCOEFT1 147,4
PAMSCOEFT2 170,8
PAMSCOEFT3 177,3
PAMSCOEFT4 167,5
PAMSCOEFT5 150,4
PAMSCOEFT6 166,2
  • Restriction digest & Gel electrophorese
  • All the 6 parts seemed to be self closed Terminator vectors.
    • The result is probably due to an error in the ligation accompanied with the low probability of taking up a large vector with the size of PAMSCOEFT (10k bp)

[edit] Vanillin Receptor

  • electropoation of virA was succesfull so it was ented
  • virB was ented from the motherplate

[edit] Key/Lock/Anti-Key

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