Team:KULeuven/Project Results

From 2009.igem.org

(Difference between revisions)
(→Project Results)
(→Project Results)
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- First and foremost we succesfully made the following parts:
- First and foremost we succesfully made the following parts:
-
Partnumber Description
+
'''Partnumber''' '''Description'''
-
K238005 Antisense key
+
{{kulpart|K238005}} Antisense key
-
K238007 Sam5 + rbs
+
{{kulpart|K238007}} Sam5 + rbs
-
K238008 virA
+
{{kulpart|K238008}} virA
-
K238009 VirG
+
{{kulpart|K238009}} VirG
-
K238011 VirB
+
{{kulpart|K238011}} VirB
-
K238013 Blue light promotor
+
{{kulpart|K238013}} Blue light promotor
-
K238014 Ech+fcs
+
{{kulpart|K238014}} Ech+fcs
-
K238015 Blp + GFP
+
{{kulpart|K238015}} Blp + GFP
-
K238016 Blp + RFP
+
{{kulpart|K238016}} Blp + RFP
-
K238017 EF + terminator
+
{{kulpart|K238017}} EF + terminator
-
K238018 EF+ promoter+ terminator
+
{{kulpart|K238018}} EF+ promoter+ terminator
-
K238019 Sam5 +sam8
+
{{kulpart|K238019}} Sam5 +sam8
-
K238020 Sams + promoter
+
{{kulpart|K238020}} Sams + promoter
-
K238021 Sams + promoter + comt
+
{{kulpart|K238021}} Sams + promoter + comt
-
K238022 Sams+ comt+ promoter en terminator
+
{{kulpart|K238022}} Sams+ comt+ promoter en terminator
-
K238024 lock
+
{{kulpart|K238024}} lock
-
K238027 Sam8 + RBS
+
{{kulpart|K238027}} Sam8 + RBS
-
K238028 Ech + rbs
+
{{kulpart|K238028}} Ech + rbs
-
K238029 Fcs + rbs
+
{{kulpart|K238029}} Fcs + rbs
All these parts were sent to the registry.  
All these parts were sent to the registry.  
-
- Secondly we tested the working of the blue light promotor:
+
The blue light promotor was characterized, the strenght of the blue light promotor was compared to a normal promotor and the effect of promotor strenght on blue light irradiance was examined.
{|
{|
-
| [[Image:weakness.png|thumb|365px|strength of our promoter in RPU]]
+
| [[Image:weakness.png|thumb|365px|Comparison of the blue light promotor (blue) to a standard promotor strength {{kulpart|I20260} (orange) in RPU. The blue light promotor shows at least half the strenght of the standard promotor, meaning it can be classified as a weak promotor]]
|}
|}
{|
{|
-
| [[Image:Light-dark.png|thumb|365px|influence of blue light on the promoter]]
+
| [[Image:Light-dark.png|thumb|365px|Effect of blue light irradiance on promotor strenght. Cells grown in blue light irradiance (blue) show a stronger expression compared to cells grown in the dark (grey)]]
|}
|}

Revision as of 14:44, 19 October 2009


Project Results

Our Results can be divided in two groups, the parts we cut and pasted together to make (test)-devices and characterizations of a part.

- First and foremost we succesfully made the following parts:

Partnumber Description

Antisense key

Sam5 + rbs

virA

VirG

VirB

Blue light promotor

Ech+fcs

Blp + GFP

Blp + RFP

EF + terminator

EF+ promoter+ terminator

Sam5 +sam8

Sams + promoter

Sams + promoter + comt

Sams+ comt+ promoter en terminator

lock

Sam8 + RBS

Ech + rbs

Fcs + rbs


All these parts were sent to the registry.

The blue light promotor was characterized, the strenght of the blue light promotor was compared to a normal promotor and the effect of promotor strenght on blue light irradiance was examined.

I20260} (orange) in RPU. The blue light promotor shows at least half the strenght of the standard promotor, meaning it can be classified as a weak promotor
Effect of blue light irradiance on promotor strenght. Cells grown in blue light irradiance (blue) show a stronger expression compared to cells grown in the dark (grey)



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