Team:UNIPV-Pavia/Methods Materials/PlasmidExtraction

From 2009.igem.org

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<h1>Plasmid extraction</h1>
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=='''Plasmid extraction'''==
''(Estimated time: 3 hours if you use QIAGEN Plasmid Mini Kit; 1 hour if you use QIAGEN QIAprep Spin Miniprep Kit)''
''(Estimated time: 3 hours if you use QIAGEN Plasmid Mini Kit; 1 hour if you use QIAGEN QIAprep Spin Miniprep Kit)''
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Revision as of 15:09, 25 June 2009

EthanolPVanimation.gif



Protocols


Plasmid extraction

(Estimated time: 3 hours if you use QIAGEN Plasmid Mini Kit; 1 hour if you use QIAGEN QIAprep Spin Miniprep Kit)

Materials needed:

  • 5 ml or 9 ml (for higher yields) overnight mini-culture
  • QIAGEN QIAprep Spin Miniprep Kit


  • Follow QIAGEN standard protocol: [http://www1.qiagen.com/literature/render.aspx?id=370&tp=4 QIAGEN QIAprep Spin Miniprep protocol]


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