Team:UNIPV-Pavia/Methods Materials/PlasmidExtraction
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- | + | =='''Plasmid extraction'''== | |
''(Estimated time: 3 hours if you use QIAGEN Plasmid Mini Kit; 1 hour if you use QIAGEN QIAprep Spin Miniprep Kit)'' | ''(Estimated time: 3 hours if you use QIAGEN Plasmid Mini Kit; 1 hour if you use QIAGEN QIAprep Spin Miniprep Kit)'' | ||
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Revision as of 15:09, 25 June 2009
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Plasmid extraction
(Estimated time: 3 hours if you use QIAGEN Plasmid Mini Kit; 1 hour if you use QIAGEN QIAprep Spin Miniprep Kit)
Materials needed:
- 5 ml or 9 ml (for higher yields) overnight mini-culture
- QIAGEN QIAprep Spin Miniprep Kit
- Follow QIAGEN standard protocol: [http://www1.qiagen.com/literature/render.aspx?id=370&tp=4 QIAGEN QIAprep Spin Miniprep protocol]