Team:LCG-UNAM-Mexico:Journals:Uriel
From 2009.igem.org
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== Agust 13, 2009 == | == Agust 13, 2009 == | ||
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+ | Plasmid 18 was dephosphorylated because we are going to insert in it the ogr product that was amplified yesterday. | ||
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+ | Dephosphorylation Reaction: | ||
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+ | Plasmid 20µL | ||
+ | Buffer 3µL | ||
+ | Enzyme 1µL | ||
+ | Water 6µL | ||
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+ | Incubation: | ||
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+ | 1)15 min --> 37ºC | ||
+ | 2)5 min --> 65ºC | ||
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+ | This plasmid that is going to be ligated with ogr is digested EcoRI/PstI | ||
== August 14, 2009 == | == August 14, 2009 == |
Revision as of 05:19, 20 October 2009