Team:Alberta/Project/AnchTermAnneal

From 2009.igem.org

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     <h1>Annealing Anchor and Terminator Bytes</h1>
     <h1>Annealing Anchor and Terminator Bytes</h1>
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Instructions on how to anneal the complementary strands to obtain the same concentrations we used in our experiments (10uM).
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Instructions on how to anneal the complementary strands to obtain the same concentrations we used in our experiments (10μM).
<h2>What you will need:</h2>
<h2>What you will need:</h2>
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<h2>Procedure:</h2>
<h2>Procedure:</h2>
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<ul>
 
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    <li>Pipet 150 uL 50% glycerol into 3* properly labeled 1.5 ml screw cap tubes.
 
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    <li>Add 350 uL of overnight culture to each tube.
 
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    <li>Pipet up and down to gently mix.
 
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    <li>Flash freeze in liquid N2 or dry ice/methanol bath
 
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    <li>Place in -80 C freezer when frozen.
 
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</ul>
 

Revision as of 05:37, 20 October 2009

University of Alberta - BioBytes










































































































Annealing Anchor and Terminator Bytes

Instructions on how to anneal the complementary strands to obtain the same concentrations we used in our experiments (10μM).

What you will need:

  • Anchor oligos (complementary)
  • Terminator oligos (complementary)

Procedure:

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