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Team:Calgary/25 June 2009
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* From the results, only one sequencing reaction was successful. The mutated site was successfully done. However, the forward and reverse reactions (both mutated and non-mutated) did not result in any sequencing. The lab is willing to repeat the sequencing reaction. | * From the results, only one sequencing reaction was successful. The mutated site was successfully done. However, the forward and reverse reactions (both mutated and non-mutated) did not result in any sequencing. The lab is willing to repeat the sequencing reaction. | ||
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Objective: Now that I have LuxOD47E with BBK sites, I need to get it into the psB1AC3 vector, as it also has the biobrick sites in it. I did this through a restriction digest of the plasmid I isolated yesterday and the psB1AC3 vector using both EcoRI/PstI and XbaI/PstI for the insert as well as both EcorI/ PstI and XbaI/PstI for the vector. I left this to digest ovenright in the 37°C waterbath. | Objective: Now that I have LuxOD47E with BBK sites, I need to get it into the psB1AC3 vector, as it also has the biobrick sites in it. I did this through a restriction digest of the plasmid I isolated yesterday and the psB1AC3 vector using both EcoRI/PstI and XbaI/PstI for the insert as well as both EcorI/ PstI and XbaI/PstI for the vector. I left this to digest ovenright in the 37°C waterbath. | ||
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Today, I called up a couple of oil and gas companies to do a follow up on our sponsorship package. Most them rejected our sponsorship proposal. I will continue to contact more companies that me and Prima found. | Today, I called up a couple of oil and gas companies to do a follow up on our sponsorship package. Most them rejected our sponsorship proposal. I will continue to contact more companies that me and Prima found. | ||
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[[Image:2009.06.24.LuxPQ_BBK_Primer_Verif+RD_Verif.png|700px]] | [[Image:2009.06.24.LuxPQ_BBK_Primer_Verif+RD_Verif.png|700px]] | ||
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On the wiki, the sponsor page is fixed. | On the wiki, the sponsor page is fixed. | ||
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I shadowed Jamie today. He showed me how to set up sequencing. HE was sending his B-R-LuxOU-B for colonies 1 and 7 to sequencing with BBK-CP-F primers. He pipetted a 10 microL of C1 and C7 in separate tubes and put the primers in a separate tube. We put primers in separately because if we had added it to the colonies, they wouldn't be able to take it out and redo it if something goes wrong. | I shadowed Jamie today. He showed me how to set up sequencing. HE was sending his B-R-LuxOU-B for colonies 1 and 7 to sequencing with BBK-CP-F primers. He pipetted a 10 microL of C1 and C7 in separate tubes and put the primers in a separate tube. We put primers in separately because if we had added it to the colonies, they wouldn't be able to take it out and redo it if something goes wrong. | ||
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Latest revision as of 06:43, 20 October 2009
UNIVERSITY OF CALGARY
