Team:Todai-Tokyo/Protocols/Ligation

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(New page: {{:Team:Todai-Tokyo/Template}} == Ligation Protocol == # Mix 2µl of insert DNA with 3µl of vector DNA # Add 5µl Takara Ligation High # Incubate at 17ºC for 30 min.)
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Revision as of 06:56, 20 October 2009

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Ligation Protocol

  1. Mix 2µl of insert DNA with 3µl of vector DNA
  2. Add 5µl Takara Ligation High
  3. Incubate at 17ºC for 30 min.
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