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Team:British Columbia/pBAD
From 2009.igem.org
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In order to make an analog biosensor, we need our traffic light to be able to respond differently to different concentrations of an input. However, the Registry is lacking in variable strength inducible promoters. We designed two variants of the <partinfo>I13453</partinfo> pBAD promoter, one weaker and one stronger, based on AraC binding experiments performed by [[Team:British_Columbia/Bibliography|Niland et al.]] | In order to make an analog biosensor, we need our traffic light to be able to respond differently to different concentrations of an input. However, the Registry is lacking in variable strength inducible promoters. We designed two variants of the <partinfo>I13453</partinfo> pBAD promoter, one weaker and one stronger, based on AraC binding experiments performed by [[Team:British_Columbia/Bibliography|Niland et al.]] | ||
| - | pbad wt ACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC | + | pbad wt ACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC<font color=red>ATTTTTA</font>TCCATAAGATTAGCGGATCCTACCTGACGCTTTTT... |
| - | pbad weak ACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC | + | pbad weak ACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC<font color=red>TTTTTTA</font>TCCATAAGATTAGCGGATCCTACCTGACGCTTTTT... |
| - | pbad strong ACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC | + | pbad strong ACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC<font color=red>AAGATAG</font>TCCATAAGATTAGCGGATCCTACCTGACGCTTTTT... |
Above is a sequence alignment of our promoter variants with the wild type pBAD, truncated for space. For the full sequences, see our parts in the registry at <partinfo>K206000</partinfo> (strong) and <partinfo>K206001</partinfo> (weak). | Above is a sequence alignment of our promoter variants with the wild type pBAD, truncated for space. For the full sequences, see our parts in the registry at <partinfo>K206000</partinfo> (strong) and <partinfo>K206001</partinfo> (weak). | ||
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| + | <html>Here is the experimental data regarding the strength of these promoters when coupled to RFP: <a href="https://static.igem.org/mediawiki/2009/e/e6/British_Columbia_pBAD_RFP%2BTimecourse%2B3.xls">pBAD_RFP_Timecourse_3.xls</a></html> | ||
Revision as of 07:37, 20 October 2009
| Home | The Team | The Traffic Light | pBAD Promoters | Lock and Key | Jammer | Parts Submitted to the Registry | Our Sponsors | Lab Notebook | Bibliography |
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Overview
In order to make an analog biosensor, we need our traffic light to be able to respond differently to different concentrations of an input. However, the Registry is lacking in variable strength inducible promoters. We designed two variants of the pBAD promoter, one weaker and one stronger, based on AraC binding experiments performed by Niland et al.
pbad wt ACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTT... pbad weak ACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCTTTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTT... pbad strong ACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCAAGATAGTCCATAAGATTAGCGGATCCTACCTGACGCTTTTT...
Above is a sequence alignment of our promoter variants with the wild type pBAD, truncated for space. For the full sequences, see our parts in the registry at (strong) and (weak).
Here is the experimental data regarding the strength of these promoters when coupled to RFP: pBAD_RFP_Timecourse_3.xls
