Team:Todai-Tokyo/Protocols/Gel Extraction

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(New page: {{:Team:Todai-Tokyo/Template}} == Gel Purification Protocol == # Cut out volume of agarose gel containing the desired DNA and put in an eppendorf tube # Use the Promega Gel Purification...)
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Gel Purification Protocol

  1. Cut out volume of agarose gel containing the desired DNA and put in an eppendorf tube
  2. Use the Promega Gel Purification kit according to instructions
  3. Elute DNA with 50µl of MilliQ (or 30µl if yield is low)
  4. Measure concentration using a spectrophotometer and write on tube