Team:Freiburg bioware/Notebook/April

From 2009.igem.org

(Difference between revisions)
Line 50: Line 50:
  class="t">Human
  class="t">Human
Practice</span></a>
Practice</span></a>
-
 
+
    <ul>
 +
      <li><a href="#">D'oh! Something's missing here.</a>
 +
        <ul>
 +
          <li><a href="#">1</a> </li>
 +
          <li><a href="#">2</a> </li>
 +
          <li><a href="#">3</a> </li>
 +
        </ul>
       </li>
       </li>
       <li><a href="#">4</a></li>
       <li><a href="#">4</a></li>
Line 59: Line 65:
  class="r"></span><span class="t">Notebook</span></a></li>
  class="r"></span><span class="t">Notebook</span></a></li>
   <li><a
   <li><a
-
href="https://2009.igem.org/Team:Freiburg_bioware/cloning1"><span
 
-
class="l"></span><span class="r"></span><span
 
-
class="t">Parts</span></a><ul>
 
-
      <li><a
 
-
href="https://2009.igem.org/Team:Freiburg_bioware/cloning1">Basic
 
-
Parts</a></li>
 
-
      <li><a
 
-
href="https://2009.igem.org/Team:Freiburg_bioware/cloning">Composite
 
-
Parts</a></li>
 
-
    </ul></li>
 
-
<li><a
 
  href="https://2009.igem.org/Team:Freiburg_bioware/Collaboration"><span
  href="https://2009.igem.org/Team:Freiburg_bioware/Collaboration"><span
  class="l"></span><span class="r"></span><span
  class="l"></span><span class="r"></span><span
Line 90: Line 85:
<div class="art-Post-inner">
<div class="art-Post-inner">
<div class="art-PostMetadataHeader">
<div class="art-PostMetadataHeader">
-
<h2 style="border-bottom: none;" class="art-PostHeaderIcon-wrapper"> &nbsp;<img
+
<h2 class="art-PostHeaderIcon-wrapper"> &nbsp;<img
  style="width: 28px; height: 25px;" alt=""
  style="width: 28px; height: 25px;" alt=""
  src="https://static.igem.org/mediawiki/2009/2/2a/Freiburg09_Post_tanne_2.png" />
  src="https://static.igem.org/mediawiki/2009/2/2a/Freiburg09_Post_tanne_2.png" />

Revision as of 10:20, 20 October 2009

FREiGEM

  April

24th April 12:30 - 18:00 o'clock Manuel

Literaturrecherche zu FokI.
Entstandene Ideen:
  • three oligo's: two for binding the monomers and one to create a dsDNA where FokI can bind.
  • two oligo's: one for binding one monomer, the second for binding the second monomer and to create dsDNA (one monomer has to be inactivated).
  • one oligo: both binding sites of the monomers on one oligo (also one monomer has to be inactivated).

The 196 C-terminale aminoacids of FokI were used from other groups (see reference 1). Primersequences are also provided in the publication.
Reference 1: Freiburg09_FokI_Zn-Finger_1996.pdf

29th april 21o'clock Rüdiger


contact:  freigem09@googlemail.com

Retrieved from "http://2009.igem.org/Team:Freiburg_bioware/Notebook/April"