"
Team:Todai-Tokyo/Protocols/Infusion
From 2009.igem.org
(Difference between revisions)
(→Infusion Protocol) |
(→Infusion Protocol) |
||
| Line 9: | Line 9: | ||
== Infusion Protocol == | == Infusion Protocol == | ||
| + | |||
| + | # Mix the purified PCR insert(50-200ng) and linearized vector(100-400ng) | ||
| + | # Add 1 | ||
Revision as of 10:31, 20 October 2009
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook | Protocols | Ethics |
|---|
Infusion Description
Infusion is a method to combine DNA fragments using a combination of PCR and homologous recombination. It can be used as an alternative to ligation with the advantage that it does not require restriction enzyme digests, although specific primers are required for the reaction.
[http://bioinfo.clontech.com/infusion/ Clonetech page on In-Fusion]
Infusion Protocol
- Mix the purified PCR insert(50-200ng) and linearized vector(100-400ng)
- Add 1
