September/29 September 2009
From 2009.igem.org
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Latest revision as of 11:13, 20 October 2009
1.colony check
sample no no. of colony 36 5 37 5 38 3 39 1 53 1
2.digation
K204057
Vector | Insert | ||
---|---|---|---|
1-23L | 7 | 35 | 2 |
EcoRI | 1 | EcoRI | 1 |
XbaI | 1 | SpeI | 1 |
No.2 | 2 | No.2 | 2 |
dH2O | 9 | dH2O | 14 |
total | 20uL | total | 20uL |
↓
37degree , 3hr
Agarose Gel Electro-phoresis
gel cut
purification
ligation
3.transformation
sample No. 52
4.colony check
sample no no. of colony 28 1 29 1 20 3 11 3 X1 4 X2 6 X3 10 X4 1 X4 6 X5 <30 X6 10 X7 2 X8 3