September/29 September 2009

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[https://2009.igem.org/Team:Osaka/NOTES back to NOTES]

Latest revision as of 11:13, 20 October 2009

1.colony check

sample no    no. of colony
  36              5
  37              5
  38              3
  39              1
  53              1


2.digation

K204057

VectorInsert
1-23L7352
EcoRI1EcoRI1
XbaI1SpeI1
No.22No.22
dH2O9dH2O14
total20uLtotal20uL


37degree , 3hr
Agarose Gel Electro-phoresis
gel cut
purification
ligation

3.transformation

sample No. 52

4.colony check

sample no    no. of colony
  28              1
  29              1
  20              3

  11              3
  X1              4
  X2              6
  X3             10
  X4              1
  X4              6
  X5             <30
  X6             10
  X7              2
  X8              3


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