Team:Kyoto/CiC/Goals
From 2009.igem.org
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Constructing prepared liposomes which can take in mitochondrial proteins from cytoplasm and confirming whether they import proteins in vitro | Constructing prepared liposomes which can take in mitochondrial proteins from cytoplasm and confirming whether they import proteins in vitro | ||
Revision as of 15:53, 20 October 2009
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- Cells in Cells
- Goals
Goals
In this "cells in cells" project, we set three subgoals.
Subgoal 1 Liposomes that can penetrate through cells membrane
Constructing liposomes that can penetrate through cells membrane and confirming whether they get into cells
Subgoal 2 Prepared liposomes which can take in mitochondrial proteins
Constructing prepared liposomes which can take in mitochondrial proteins from cytoplasm and confirming whether they import proteins in vitro
Subgoal 3 Introducing prepared liposomes into host cells
Introducing prepared liposomes into host cells and confirming whether they take in mitochondrial proteins form cytoplasm in vivo
Future Goals
Well-prepared Liposomes
Construction of double membraned liposomes with all important mitochondrial translocases.
Artificial Mitochondrial Genome
Construction of prepared liposome (or well-prepared liposomes) which have DNA in mitochondrial format.
Chloroplasts
Reconstruction of chloroplasts in similar methods.