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Team:Todai-Tokyo/Protocols/Restriction Enzyme Digest
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== Restriction Enzyme Digest Protocol == | == Restriction Enzyme Digest Protocol == | ||
Latest revision as of 16:29, 20 October 2009
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Restriction Enzyme Digest Protocol
1. Mix the following in a eppendorf tube:
- Xµl plasmid DNA （to a final concentration of 300ng/30ul）
- 3µl 10 H buffer
- 1.5µl Restriction Enzyme 1
- (1.5µl Restriction Enzyme 2, if applicable)
- MilliQ up to 30ul
2. Incubate at 37ºC for 1h or more
