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Team:USTC/Standard & Protocol
From 2009.igem.org
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| + | ==Standard protocol== | ||
| + | ===Minipreps=== | ||
| + | Performed with BIO BASIC INC. EZ-10 Spin Column Plasmid DNA MiniPreps Kit BS414 | ||
| + | ===Digestion=== | ||
| + | The digestion enzymes we use are listed: | ||
| + | <center> | ||
| + | Pst I Fermentas ER0611 3000U | ||
| + | |||
| + | EcoR I Fermentas ER0271 5000U | ||
| + | |||
| + | Spe I Fermentas ER1251 1500U | ||
| + | |||
| + | Xba I Fermentas ER0681 400U | ||
| + | ===Gel Extraction=== | ||
| + | ===Ligation=== | ||
| + | ===Transformation=== | ||
| + | ===Colony PCR=== | ||
Revision as of 16:42, 20 October 2009
| Home | Team | Project | Modeling | Parts | Standard & Protocol | Software Tool | Human Practice | Notebook |
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Team:USTC/Standard & Protocol
Contents |
Standard protocol
Minipreps
Performed with BIO BASIC INC. EZ-10 Spin Column Plasmid DNA MiniPreps Kit BS414
Digestion
The digestion enzymes we use are listed:
Pst I Fermentas ER0611 3000U
EcoR I Fermentas ER0271 5000U
Spe I Fermentas ER1251 1500U
Xba I Fermentas ER0681 400U
