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Team:USTC/Standard & Protocol
From 2009.igem.org
(Difference between revisions)
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Performed with BIO BASIC INC. EZ-10 Spin Column DNA Gel Extraction Kit BS354 | Performed with BIO BASIC INC. EZ-10 Spin Column DNA Gel Extraction Kit BS354 | ||
===Ligation=== | ===Ligation=== | ||
| + | For short segments: | ||
| + | |||
| + | TakaRa DNA Ligation Kit Ver2.0 Code D6022 | ||
| + | |||
| + | BIO BASIC INC. FAST LIGATION KIT BS512 | ||
| + | |||
| + | For long segments ligation: | ||
| + | |||
| + | TaKaRa DNA Ligation Kit LONG Code D6024 | ||
===Transformation=== | ===Transformation=== | ||
===Colony PCR=== | ===Colony PCR=== | ||
Revision as of 16:52, 20 October 2009
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Team:USTC/Standard & Protocol
Contents |
Standard protocol
Minipreps
Performed with BIO BASIC INC. EZ-10 Spin Column Plasmid DNA MiniPreps Kit BS414
Digestion
The digestion enzymes we use are listed:
Pst I Fermentas ER0611 3000U
EcoR I Fermentas ER0271 5000U
Spe I Fermentas ER1251 1500U
Xba I Fermentas ER0681 400U
Gel Extraction
Performed with BIO BASIC INC. EZ-10 Spin Column DNA Gel Extraction Kit BS354
Ligation
For short segments:
TakaRa DNA Ligation Kit Ver2.0 Code D6022
BIO BASIC INC. FAST LIGATION KIT BS512
For long segments ligation:
TaKaRa DNA Ligation Kit LONG Code D6024
