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Team:USTC/Standard & Protocol
From 2009.igem.org
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===Colony PCR=== | ===Colony PCR=== | ||
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Revision as of 16:57, 20 October 2009
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Team:USTC/Standard & Protocol
Contents |
Standard protocol
Minipreps
Performed with BIO BASIC INC. EZ-10 Spin Column Plasmid DNA MiniPreps Kit BS414
Digestion
The digestion enzymes we use are listed:
Pst I Fermentas ER0611 3000U
EcoR I Fermentas ER0271 5000U
Spe I Fermentas ER1251 1500U
Xba I Fermentas ER0681 400U
Gel Extraction
Performed with BIO BASIC INC. EZ-10 Spin Column DNA Gel Extraction Kit BS354
Ligation
For short segments:
TakaRa DNA Ligation Kit Ver2.0 Code D6022
BIO BASIC INC. FAST LIGATION KIT BS512
For long segments ligation:
TaKaRa DNA Ligation Kit LONG Code D6024
