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Team:Imperial College London/Wetlab/Results/Cheminduction
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Different concentrations of IPTG were added to a 96-well plate [link to assay] of e-coli Top 10 cells containing LacI-RFP genetic constructs. Here we measured the optical density of the cells over-time, for different concentrations of IPTG to monitor the effects in the culture. The IPTG growth curve results are plotted below:<br> | Different concentrations of IPTG were added to a 96-well plate [link to assay] of e-coli Top 10 cells containing LacI-RFP genetic constructs. Here we measured the optical density of the cells over-time, for different concentrations of IPTG to monitor the effects in the culture. The IPTG growth curve results are plotted below:<br> | ||
[[Image:II09_IPTG_growth1.jpg]]<br> | [[Image:II09_IPTG_growth1.jpg]]<br> | ||
| - | <b>Figure 1: Effects of [IPTG] on cell growth</b><br> | + | <b>Figure 1: Effects of [IPTG] on cell growth</b><br>: Note that the high initial OD value results due to cultures having grown for 1 hour prior the start of the measuring phase. |
{{Imperial/09/TemplateBottom}} | {{Imperial/09/TemplateBottom}} | ||
Revision as of 20:11, 20 October 2009
Experiment:
Different concentrations of IPTG were added to a 96-well plate [link to assay] of e-coli Top 10 cells containing LacI-RFP genetic constructs. Here we measured the optical density of the cells over-time, for different concentrations of IPTG to monitor the effects in the culture. The IPTG growth curve results are plotted below:
Figure 1: Effects of [IPTG] on cell growth
: Note that the high initial OD value results due to cultures having grown for 1 hour prior the start of the measuring phase.
