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Team:Imperial College London/Wetlab/Results/Cheminduction
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[[Image:II09_IPTG_growth1.jpg]]<br> | [[Image:II09_IPTG_growth1.jpg]]<br> | ||
<b>Figure 1: Effects of [IPTG] on cell growth</b>: Note that the high initial OD value results due to cultures having grown for 1 hour prior the start of the measuring phase.<br> | <b>Figure 1: Effects of [IPTG] on cell growth</b>: Note that the high initial OD value results due to cultures having grown for 1 hour prior the start of the measuring phase.<br> | ||
| + | <br> | ||
| + | Simply plotting the raw data does not give sufficient information about possible trends. | ||
| + | Therefore, further analysis was performed where: | ||
| + | 1. The blank absorbance level was estimated | ||
| + | [[Image:II09_chemo1_blank_variation.png]] | ||
| + | |||
{{Imperial/09/TemplateBottom}} | {{Imperial/09/TemplateBottom}} | ||
Revision as of 20:17, 20 October 2009
Experiment:
Different concentrations of IPTG were added to a 96-well plate [link to assay] of e-coli Top 10 cells containing LacI-RFP genetic constructs. Here we measured the optical density of the cells over-time, for different concentrations of IPTG to monitor the effects in the culture. The IPTG growth curve results are plotted below:
Figure 1: Effects of [IPTG] on cell growth: Note that the high initial OD value results due to cultures having grown for 1 hour prior the start of the measuring phase.
Simply plotting the raw data does not give sufficient information about possible trends.
Therefore, further analysis was performed where:
1. The blank absorbance level was estimated
