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Team:Kyoto/CiC/Goals
From 2009.igem.org
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Our third subgoal is introducing prepared liposomes into host cells and confirming whether they take in mitochondrial proteins form cytoplasm in vivo.This subgoal requires both of subgoals above. | Our third subgoal is introducing prepared liposomes into host cells and confirming whether they take in mitochondrial proteins form cytoplasm in vivo.This subgoal requires both of subgoals above. | ||
Revision as of 21:22, 20 October 2009
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- Cells in Cells
- Goals
Goals
In this "cells in cells" project, we set three subgoals.
Subgoal A --- Liposomes that can penetrate through cell membrane
Our first subgoal is construction of liposomes that can penetrate through cell membrane and confirming whether they get into cells.
Subgoal B --- Prepared liposomes which can take in mitochondrial proteins
Our second subgoal is construction of prepared liposomes which can take in mitochondrial proteins from cytoplasm and confirming whether they import proteins in vitro.
Subgoal C --- In Vivo Protein Import into Prepared Liposome
Our third subgoal is introducing prepared liposomes into host cells and confirming whether they take in mitochondrial proteins form cytoplasm in vivo.This subgoal requires both of subgoals above.
Future Goals
Well-prepared Liposomes
Construction of double membraned liposomes with all important mitochondrial translocases.
Artificial Mitochondrial Genome
Construction of prepared liposome (or well-prepared liposomes) which have DNA in mitochondrial format.
Chloroplasts
Reconstruction of chloroplasts in similar methods.
